Synthetic libraries of shark vNAR domains with different cysteine numbers within the CDR3.

ABSTRACT: The variable domain of New Antigen Receptors (vNAR) from sharks, present special characteristics in comparison to the conventional antibody molecules such as: small size (12-15 kDa), thermal and chemical stability and great tissue penetration, that makes them a good alternative source as therapeutic or diagnostic agents. Therefore, it is essential to improve techniques used for the development and selection of vNAR antibodies that recognize distinct antigens. The development of synthetic antibody libraries offers a fast option for the generation of antibodies with the desired characteristics. In this work three synthetic antibody libraries were constructed; without cysteines (Cys), with one Cys and with two Cys residues within its CDR3, with the objective of determining whether the presenc

SUBMITTER: Cabanillas-Bernal O 

PROVIDER: S-EPMC6576789 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature