Integrating a generalized data analysis workflow with the Single-probe mass spectrometry experiment for single cell metabolomics.

ABSTRACT: We conducted single cell metabolomics studies of live cancer cells through online single cell mass spectrometry (SCMS) experiments combined with a generalized comprehensive data analysis workflow. The SCMS experiments were carried out using the Single-probe device coupled with a mass spectrometer to measure molecular profiles of cells in response to two mitotic inhibitors, taxol and vinblastine, under a series of treatment conditions. SCMS metabolomic data were analyzed using a comprehensive approach, including data pre-treatment, visualization, statistical analysis, machine learning, and pathway enrichment analysis. For comparative studies, traditional liquid chromatography-MS (LC-MS) experiments were conducted using lysates prepared from bulk cell samples. Metabolomic profiles of single

SUBMITTER: Liu R 

PROVIDER: S-EPMC6579046 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature