Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

ABSTRACT: Human erythropoiesis serves as a paradigm of physiologic cellular differentiation. This process is also of considerable interest for better understanding anemias and identifying new therapies. Here, we apply deep transcriptomic and accessible chromatin profiling to characterize a faithful ex vivo human erythroid differentiation system from hematopoietic stem and progenitor cells. We reveal stage-specific transcriptional states and chromatin accessibility during various stages of erythropoiesis, including 14,260 differentially expressed genes and 63,659 variably accessible chromatin peaks. Our analysis suggests differentiation stage-predominant roles for specific master regulators, including GATA1 and KLF1. We integrate chromatin profiles with common and rare genetic variants associated wit

SUBMITTER: Ludwig LS 

PROVIDER: S-EPMC6579117 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature