The quantitative relationship between isotopic and net contributions of lactate and glucose to the tricarboxylic acid (TCA) cycle.

ABSTRACT: Whether growing cancer cells prefer lactate as a fuel over glucose or vice versa is an important but controversial issue. Labeling of tricarboxylic acid (TCA) cycle intermediates with glucose or lactate isotope tracers is often used to report the relative contributions of these two metabolites to the TCA cycle. However, this approach may not yield accurate results, as isotopic labeling may not accurately reflect net contributions of each metabolite. This may be due to isotopic exchange occurring during the conversion between pyruvate and lactate. To evaluate this quantitatively, we used an equation (C _G - C _G' = C _L' - C _L) assessing the relationship between isotopic labeling and net consumption measurements in vitr

SUBMITTER: Ying M 

PROVIDER: S-EPMC6579476 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature