Gene expression analysis of conjunctival epithelium of patients with Stevens-Johnson syndrome in the chronic stage.

ABSTRACT:

Objective

To investigate the pathology underlying the ocular surface complications of patients with Stevens-Johnson syndrome (SJS) in the chronic stage.

Methods and analysis

Using oligonucleotide microarrays, we performed comprehensive gene expression analysis of the conjunctival epithelium of patients with SJS in the chronic stage (n=3). The controls were patients with conjunctival chalasis (n=3). We confirmed the downregulation and upregulation of transcripts of interest by quantitative real-time PCR (RT-PCR) assay. The expression of ocular surface protein with significantly upregulated transcripts was assessed immunohistochemically.

Results

Compared with the controls, in the conjunctival epithelium of patients with SJS, 50 transcripts were downregulated by less tha

SUBMITTER: Ueta M 

PROVIDER: S-EPMC6579564 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature