A single cell transcriptional portrait of embryoid body differentiation and comparison to progenitors of the developing embryo.

ABSTRACT: Directed differentiation of pluripotent stem cells provides an accessible system to model development. However, the distinct cell types that emerge, their dynamics, and their relationship to progenitors in the early embryo has been difficult to decipher because of the cellular heterogeneity inherent to differentiation. Here, we used a combination of bulk RNA-Seq, single cell RNA-Seq, and bioinformatics analyses to dissect the cell types that emerge during directed differentiation of mouse embryonic stem cells as embryoid bodies and we compared them to spatially and temporally resolved transcriptional profiles of early embryos. Our single cell analyses of the day 4 embryoid bodies revealed three populations which had retained related yet distinct pluripotent signatures that resemble the pre

SUBMITTER: Spangler A 

PROVIDER: S-EPMC6579609 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature