ITRAQ‑based proteomic analysis of endotoxin tolerance induced by lipopolysaccharide.

ABSTRACT: The purpose of the present study was to investigate the differentially expressed proteins between endotoxin tolerance and sepsis. Cell models of an endotoxin tolerance group (ET group) and sepsis group [lipopolysaccharide (LPS) group] were established using LPS and evaluated using ELISA and flow cytometry methods. Differentially expressed proteins between the ET and the LPS groups were identified using isobaric tags for relative and absolute quantitation (iTRAQ) analysis and evaluated by bioinformatics analysis. The expression of core proteins was detected by western blotting. It was identified that the expression of tumor necrosis factor‑α and interleukin‑6 was significantly decreased in the ET group compared with the LPS group. Following high‑dose LPS stimulation for 24 h, the positive r

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC6580005 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature