Elevated expression of TREK-TRAAK K_2P channels in the retina of adult rd1 mice.

ABSTRACT:

Aim

To examine the expression of Twik-related K⁺ channel 1 (TREK-1), Twik-related K⁺ channel 2 (TREK-2), and Twik-related arachidonic acid-stimulated K⁺ channel (TRAAK) in the retina of adult rd1 mice and to detect the protective roles of TREK-TRAAK two-pore-domain K⁺ (K_2P) channels against retinal degeneration.

Methods

Twenty-eight-day-old C57BL/6J mice and 28-day-old rd1 mice were used in this study. Retinal protein, retinal RNA, and embedded eyeballs were prepared from these two groups of mice. Real-time quantitative polymerase chain reaction and Western blot analyses were used to assess the gene transcription and protein levels, respectively. Retinal structures were observed using hematoxylin and eosin (H&E) staining

SUBMITTER: Zhang XT 

PROVIDER: S-EPMC6580209 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature