Unveiling the Importance of Amide Protons in CSP:ComD Interactions in Streptococcus pneumoniae.

ABSTRACT: Streptococcus pneumoniae is an opportunistic pathogen that can cause diseases ranging from mild respiratory infections to life-threatening conditions such as pneumonia, meningitis, and bacteremia. S. pneumoniae pathogenicity is dependent on the action of a 17-amino acid peptide pheromone, termed competence stimulating peptide (CSP) that controls the competence regulon, a quorum sensing (QS) circuit. Therefore, intercepting QS could have therapeutic implications in treating pneumococcal infections while avoiding emerging antimicrobial resistance. In this study, we set out to evaluate the impact of amide protons on CSP activity and metabolic stability through systematic N-methylation. Our results indicate that the majority of amide protons are critical for CSP activity,

SUBMITTER: Koirala B 

PROVIDER: S-EPMC6580532 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature