Defining housekeeping genes suitable for RNA-seq analysis of the human allograft kidney biopsy tissue.

ABSTRACT:

Background

RNA-seq is poised to play a major role in the management of kidney transplant patients. Rigorous definition of housekeeping genes (HKG) is essential for further progress in this field. Using single genes or a limited set HKG is inherently problematic since their expression might be altered by specific diseases in the patients being studied.

Methods

To generate a HKG set specific for kidney transplantation, we performed RNA-sequencing from renal allograft biopsies collected in a variety of clinical settings. Various normalization methods were applied to identify transcripts that had a coefficient of variation of expression that was below the 2nd percentile across all samples, and the corresponding genes were designated as housekeeping genes. Comparison with transcr

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6580566 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature