HIV-1 subtype C Envelope function becomes less sensitive to N-glycosylation deletion during disease progression.

ABSTRACT:

Objective

As part of a larger study to understand how Envelope N-glycosylation influences HIV-1 pathogenesis, we selected a participant infected with a single Subtype C variant and determined whether deletion of specific potential N-glycan sites (PNGs) impacted Envelope function longitudinally.

Results

We deleted five PNGs previously linked to HIV-1 transmission of two matched Envelope clones representing variants at 5 and 173 weeks post-infection. The transmitted founder (TF) had significantly better pseudovirus entry efficiency than the chronic infection (CI) variant. Deletion of all PNGs significantly reduced TF entry efficiency, binding to dendritic cell-specific intracellular adhesion molecule 3 grabbing non-integrin (DC-SIGN) receptor and trans-infection. However, muta

SUBMITTER: Lumngwena EN 

PROVIDER: S-EPMC6580609 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature