The involvement of spinal annexin A10/NF-κB/MMP-9 pathway in the development of neuropathic pain in rats.

ABSTRACT:

Background

Neuropathic pain (NP) is a prevalent disease, which badly impairs the life quality of patients. The underlying mechanism of NP is still not fully understood. It has been reported that spinal Annexin A10 (ANXA10) contributes to NP. This study aims at exploring the underlying mechanisms of spinal ANXA10 in regulating NP in rats.

Methods

Spinal nerve ligation (SNL) was adopted to establish a NP model in rats. After SNL, paw withdrawal threshold and paw withdrawal latency were recorded to measure pain behaviors, RT-PCR was used to check the change of the expression of spinal ANXA10 mRNA, western blot analysis was used to detect the change of the protein level of ANXA10, nuclear factor kappa B (NF-κB), and maisrix metalloproteinase-9 (MMP-9) in the spinal cord. The lev

SUBMITTER: Sun L 

PROVIDER: S-EPMC6580616 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature