Single-cell RNAseq reveals seven classes of colonic sensory neuron.

ABSTRACT:

Objective

Integration of nutritional, microbial and inflammatory events along the gut-brain axis can alter bowel physiology and organism behaviour. Colonic sensory neurons activate reflex pathways and give rise to conscious sensation, but the diversity and division of function within these neurons is poorly understood. The identification of signalling pathways contributing to visceral sensation is constrained by a paucity of molecular markers. Here we address this by comprehensive transcriptomic profiling and unsupervised clustering of individual mouse colonic sensory neurons.

Design

Unbiased single-cell RNA-sequencing was performed on retrogradely traced mouse colonic sensory neurons isolated from both thoracolumbar (TL) and lumbosacral (LS) dorsal root ganglia associated w

SUBMITTER: Hockley JRF 

PROVIDER: S-EPMC6580772 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature