Awake ¹⁸F-FDG PET Imaging of Memantine-Induced Brain Activation and Test-Retest in Freely Running Mice.

ABSTRACT: PET scans of the mouse brain are usually performed with anesthesia to immobilize the animal. However, it is desirable to avoid the confounding factor of anesthesia in mouse-brain response. Methods: We developed and validated brain PET imaging of awake, freely moving mice. Head-motion tracking was performed using radioactive point-source markers, and we used the tracking information for PET-image motion correction. Regional ¹⁸F-FDG brain uptake in a test, retest, and memantine-challenge study was measured in awake (n = 8) and anesthetized (n = 8) C57BL/6 mice. An awake uptake period was considered for the anesthesia scans. Results: Awake (motion-corrected) PET images showed an ¹⁸F-FDG uptake pattern comparable to the pattern of anesthetized

SUBMITTER: Miranda A 

PROVIDER: S-EPMC6581220 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature