Immuno-PET of Innate Immune Markers CD11b and IL-1β Detects Inflammation in Murine Colitis.

ABSTRACT: Inflammatory bowel disease (IBD) is a chronic relapsing and remitting inflammatory disease of the gastrointestinal tract. The diagnosis and monitoring of IBD are reliant on endoscopy, which is invasive and does not provide information on specific mediators. Symptom flare in IBD is associated with increased activation of innate immune pathways. Immuno-PET approaches have previously demonstrated the ability to detect colitis; however, a direct comparison of antibodies targeted to innate immune mediators and cells has not been done. We aimed to compare immuno-PET of antibodies to IL-1β and CD11b against standard ¹⁸F-FDG and MRI approaches to detect colonic inflammation. Methods: Colonic concentrations of IL-1β and myeloperoxidase were determined by ELISA, and colonic infiltr

SUBMITTER: Dmochowska N 

PROVIDER: S-EPMC6581233 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature