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The correlation between expression of sip protein in different serotypes of group b streptococcus and diagnosis.

ABSTRACT: Since the surface immunogenic protein (Sip) of group B streptococcus was identified, it's immunogenicity and its potential as a universal vaccine candidate has been evaluated extensively. We developed recombinant Sip protein and used it for monoclonal antibody generation to develop immunochromatographic test kit for GBS detection. The test of bacteria and culture media revealed the correlation between Sip protein expression and diagnosis discrepancy, which has never been reported. Furthermore, not only the surface accessibility of the Sip protein may vary from strains or serotypes; the secretion level of Sip protein may also vary.

SUBMITTER: Cheng S

PROVIDER: S-EPMC6581873 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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The correlation between expression of sip protein in different serotypes of group b streptococcus and diagnosis.

Cheng Shiliang S Han Jiae J Huang Yidan Y Yan Qin Q Lu Gang G Yuan Zuowei Z Huang Guanjun G Zheng Jian J Liu Tianqiang T

Heliyon 20190612 6

Since the surface immunogenic protein (Sip) of group B streptococcus was identified, it's immunogenicity and its potential as a universal vaccine candidate has been evaluated extensively. We developed recombinant Sip protein and used it for monoclonal antibody generation to develop immunochromatographic test kit for GBS detection. The test of bacteria and culture media revealed the correlation between Sip protein expression and diagnosis discrepancy, which has never been reported. Furthermore, n ...[more]

PMID: 31245642

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Project description:Aim: To find the genes that related with the drug's(7-P) function. Design: 36 Rats were randomly divided into three group:control group(12), model group(12) and drug group(12). At last, randomly choosing 3 Rat from each group to do genechip analysis. In model group, the Porcine serum was given intraperitoneally (i.p.) to rats in a volume of 1.5 mL/kg every 3 days for a total of 20 treatments. Effect on model animals was confirmed by liver histology and biochemical assays . In drug group, 7-P was dissolved in balanced saline solution for administration subcutaneously at dosages of 174 μg/kg in a volume of 1 mL/kg. The doses were given 15 times, one day interval. In control group, the animals were not treated with porcine serum. but given an equal volume of vehicle alone. During 7-P and vehicle administration, porcine serum was given intraperitoneally (i.p.) to rats in a volume of 1.5 mL/ kg once weekly. All animals were fasted for 24 h before porcine serum treatment. Background: During investigation of hypervariable region 1 (HVR1) of hepatitis C virus (HCV), a seven amino acids mimic peptide named 7-P was identified within the N-terminal of HVR1 that could induce IFN-γ and IL-10 expression while decreasing the expression of TNF-α in vivo and in vitro. Further studies revealed that 7-P could not only decrease the serum levels of aminotransferases (ALT, AST), alkaline phosphatase (AKP), and billirubin, but were also effective in reversing histopathological evidence of inflammation-mediated hepatic injury in 3 rat models. The protective mechanism and gene expression profiles were investigated in detail in a rat chronic liver injury model using Affymetrix microarrays . Conclusion: We found that IL-1β, tumor necrosis factor receptor superfamily 14 (TNFRSF14), intercellular adhesion molecule 2 (ICAM2), sterol regulatory element-binding protein 1 (SREBP 1), Prohibitin (PHB), and inhibitor of DNA binding 2 (Id2) were the target genes that related with 7-P Keywords: comparative genomic hybridization

Dataset Information

The correlation between expression of sip protein in different serotypes of group b streptococcus and diagnosis.

Publications

The correlation between expression of sip protein in different serotypes of group b streptococcus and diagnosis.

Similar Datasets

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