Development of a Rhinovirus Inoculum Using a Reverse Genetics Approach.

ABSTRACT:

Background

Experimental inoculation is an important tool for common cold and asthma research. Producing rhinovirus (RV) inocula from nasal secretions has required prolonged observation of the virus donor to exclude extraneous pathogens. We produced a RV-A16 inoculum using reverse genetics and determined the dose necessary to cause moderate colds in seronegative volunteers.

Methods

The consensus sequence of RV-A16 from a previous inoculum was cloned, and inoculum virus was produced using reverse genetics techniques. After safety testing, volunteers were inoculated with either RV-A16 (n = 26) or placebo (n = 10), Jackson cold scores were recorded, and nasal secretions were tested for shedding of RV-A16 ribonucleic acid.

Results

The reverse genetics process produced infe

SUBMITTER: Gern JE 

PROVIDER: S-EPMC6581892 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature