LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion.

ABSTRACT: The mechanism of action of various viruses has been the primary focus of many studies. Yet, the data on RNA modifications in any type of virus are scarce. Methods for the sensitive analysis of RNA modifications have been developed only recently and they have not been applied to viruses. In particular, the RNA composition of HIV-1 virions has never been determined with sufficiently exact methods. Here, we reveal that the RNA of HIV-1 virions contains surprisingly high amount of the 1-methyladenosine. We are the first to use a liquid chromatography-mass spectrometry analysis (LC/MS) of virion RNA, which we combined with m¹A profiling and deep sequencing. We found that m¹A was present in the tRNA, but not in the genomic HIV-1 RNA and the abundant 7SL RNA. We were able to

SUBMITTER: Simonova A 

PROVIDER: S-EPMC6581912 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature