Transcriptome Responses to Dexamethasone Depending on Dose and Glucocorticoid Receptor Sensitivity in the Liver.

ABSTRACT: Tissue sensitivity to glucocorticoids is a key factor dictating outcome of their homeostatic and therapeutic action, whereby liver represents one of the major peripheral targets. Here, we used pigs carrying a natural gain-of-function glucocorticoid receptor (GR) variant Ala610Val (GR_Ala610Val) as a model to identify genes and pathways related to differential glucocorticoid sensitivity. Animals with different GR_Ala610Val genotypes were treated either with saline or two different doses of dexamethasone. Genome-wide transcriptional responses depending on treatment, genotype, and their interaction in the liver were investigated using mRNA sequencing. Dexamethasone induced vast transcriptional responses, with more than 30% of present genes being affected. Functional annota

SUBMITTER: Murani E 

PROVIDER: S-EPMC6582245 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature