Bacterial genome architecture shapes global transcriptional regulation by DNA supercoiling.

ABSTRACT: DNA supercoiling acts as a global transcriptional regulator in bacteria, that plays an important role in adapting their expression programme to environmental changes, but for which no quantitative or even qualitative regulatory model is available. Here, we focus on spatial supercoiling heterogeneities caused by the transcription process itself, which strongly contribute to this regulation mode. We propose a new mechanistic modeling of the transcription-supercoiling dynamical coupling along a genome, which allows simulating and quantitatively reproducing in vitro and in vivo transcription assays, and highlights the role of genes' local orientation in their supercoiling sensitivity. Consistently with predictions, we show that chromosomal relaxation artificially induced by gyrase inhibitors s

SUBMITTER: El Houdaigui B 

PROVIDER: S-EPMC6582348 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature