Expression analysis of the osteoarthritis genetic susceptibility mapping to the matrix Gla protein gene MGP.

ABSTRACT:

Background

Osteoarthritis (OA) is a common disease of older individuals that impacts detrimentally on the quality and the length of life. It is characterised by the painful loss of articular cartilage and is polygenic and multifactorial. Genome-wide association scans have highlighted over 90 osteoarthritis genetic signals, some of which reside within or close to highly plausible candidate genes. An example is an association to polymorphisms within and adjacent to the matrix Gla protein gene MGP. We set out to undertake a functional study of this gene.

Methods

Nucleic acid was extracted from cartilage, infrapatellar fat pad, synovium, trabecular bone, trapezium and peripheral whole blood from OA patients and also from mesenchymal stem cells (MSCs) subjected to chondrogenesis.

SUBMITTER: Shepherd C 

PROVIDER: S-EPMC6582465 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature