DNA N6-Methyladenosine modification role in transmitted variations from genomic DNA to RNA in Herrania umbratica.

ABSTRACT:

Background

DNA methylation is an important epigenetic modification. Recently the developed single-molecule real-time (SMRT) sequencing technology provided an efficient way to detect DNA N6-methyladenine (6mA) modification that played an important role in epigenetic and positively regulated gene expression. In addition, the gene expression was also regulated by genetic variation. However, the relationship between DNA 6mA modification and variation is still unknown.

Results

We collected the SMRT long-reads DNA, Illumina short reads DNA and RNA datasets from the young leaves of Herrania umbratica, and used them to detect 35,654 6mA modification sites, 829,894 DNA variations and 60,672 RNA variations respectively, among which, there are 303 DNA variations and 19 RNA variations w

SUBMITTER: Luan MW 

PROVIDER: S-EPMC6582544 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature