Engineered mosaic protein polymers; a simple route to multifunctional biomaterials.

ABSTRACT:

Background

Engineered living materials (ELMs) are an exciting new frontier, where living organisms create highly functional materials. In particular, protein ELMs have the advantage that their properties can be manipulated via simple molecular biology. Caf1 is a protein ELM that is especially attractive as a biomaterial on account of its unique combination of properties: bacterial cells export it as a massive, modular, non-covalent polymer which is resistant to thermal and chemical degradation and free from animal material. Moreover, it is biologically inert, allowing the bioactivity of each 15 kDa monomeric Caf1 subunit to be specifically engineered by mutagenesis and co-expressed in the same Escherichia coli cell to produce a mixture of bioactive Caf1 subunits.

Results<

SUBMITTER: Peters DT 

PROVIDER: S-EPMC6582577 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature