Structural Characterization of Glycosaminoglycan Carbohydrates Using Ultraviolet Photodissociation.

ABSTRACT: Structural characterization of sulfated glycosaminoglycans (GAGs) by mass spectrometry has long been a formidable analytical challenge owing to their high structural variability and the propensity for sulfate decomposition upon activation with low-energy ion activation methods. While derivatization and complexation workflows have aimed to generate informative spectra using low-energy ion activation methods, alternative ion activation methods present the opportunity to obtain informative spectra from native GAG structures. Both electron- and photon-based activation methods, including electron detachment dissociation (EDD), negative electron transfer dissociation (NETD), and extreme ultraviolet photon activation, have been explored previously to overcome the limitations associated with low-e

SUBMITTER: Klein DR 

PROVIDER: S-EPMC6582656 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature