Population-level analysis of Blastocystis subtype prevalence and variation in the human gut microbiota.

ABSTRACT:

Objective

Human gut microbiome studies are mainly bacteria- and archaea-oriented, overlooking the presence of single-cell eukaryotes such as Blastocystis, an enteric stramenopiles with worldwide distribution. Here, we surveyed the prevalence and subtype variation of Blastocystis in faecal samples collected as part of the Flemish Gut Flora Project (FGFP), a Western population cohort. We assessed potential links between Blastocystis subtypes and identified microbiota-host covariates and quantified microbiota differentiation relative to subtype abundances.

Design

We profiled stool samples from 616 healthy individuals from the FGFP cohort as well as 107 patients with IBD using amplicon sequencing targeting the V4 variable region of the 16S rRNA and 18S rRNA

SUBMITTER: Tito RY 

PROVIDER: S-EPMC6582744 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature