SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM.

ABSTRACT: Selecting particles from digital micrographs is an essential step in single-particle electron cryomicroscopy (cryo-EM). As manual selection of complete datasets-typically comprising thousands of particles-is a tedious and time-consuming process, numerous automatic particle pickers have been developed. However, non-ideal datasets pose a challenge to particle picking. Here we present the particle picking software crYOLO which is based on the deep-learning object detection system You Only Look Once (YOLO). After training the network with 200-2500 particles per dataset it automatically recognizes particles with high recall and precision while reaching a speed of up to five micrographs per second. Further, we present a general crYOLO network able to pick from previously unseen datasets, allowin

SUBMITTER: Wagner T 

PROVIDER: S-EPMC6584505 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature