3D printed micro-scale force gauge arrays to improve human cardiac tissue maturation and enable high throughput drug testing.

ABSTRACT: Human induced pluripotent stem cell - derived cardiomyocytes (iPSC-CMs) are regarded as a promising cell source for establishing in-vitro personalized cardiac tissue models and developing therapeutics. However, analyzing cardiac force and drug response using mature human iPSC-CMs in a high-throughput format still remains a great challenge. Here we describe a rapid light-based 3D printing system for fabricating micro-scale force gauge arrays suitable for 24-well and 96-well plates that enable scalable tissue formation and measurement of cardiac force generation in human iPSC-CMs. We demonstrate consistent tissue band formation around the force gauge pillars with aligned sarcomeres. Among the different maturation treatment protocols we explored, 3D aligned cultures on force gauge arrays with

SUBMITTER: Ma X 

PROVIDER: S-EPMC6584548 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature