Development of molecular assays to detect target-site mechanisms associated with insecticide resistance in malaria vectors from Latin America.

ABSTRACT:

Background

Malaria remains an important public health problem in Latin America, and the development of insecticide resistance in malaria vectors poses a major threat to malaria elimination efforts. Monitoring of insecticide susceptibility and the determination of the mechanisms involved in insecticide resistance are needed to effectively guide the deployment of appropriate vector control measures. Here, molecular assays have been developed to screen for mutations associated with insecticide resistance on the voltage-gated sodium channel (VGSC) and acetylcholinesterase-1 (Ace-1) genes in four malaria vectors from Latin America.

Methods

Degenerate primers were designed to amplify a partial fragment on the VGSC and Ace-1 genes. Wild-caught individuals for Anopheles albimanus (a

SUBMITTER: Lol JC 

PROVIDER: S-EPMC6585033 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature