Lung adenocarcinoma-intrinsic GBE1 signaling inhibits anti-tumor immunity.

ABSTRACT:

Background

Changes in glycogen metabolism is an essential feature among the various metabolic adaptations used by cancer cells to adjust to the conditions imposed by the tumor microenvironment. Our previous study showed that glycogen branching enzyme (GBE1) is downstream of the HIF1 pathway in hypoxia-conditioned lung cancer cells. In the present study, we investigated whether GBE1 is involved in the immune regulation of the tumor microenvironment in lung adenocarcinoma (LUAD).

Methods

We used RNA-sequencing analysis and the multiplex assay to determine changes in GBE1 knockdown cells. The role of GBE1 in LUAD was evaluated both in vitro and in vivo.

Results

GBE1 knockdown increased the expression of chemokines CCL5 and CXCL10 in A549 cells. CD8 expression correlated

SUBMITTER: Li L 

PROVIDER: S-EPMC6585057 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature