EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer.

ABSTRACT:

Background

Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk.

Methods

In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-assoc

SUBMITTER: Roman-Canal B 

PROVIDER: S-EPMC6585099 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature