Use of autologous ^99mTechnetium-labelled neutrophils to quantify lung neutrophil clearance in COPD.

ABSTRACT:

Rationale

There is a need to develop imaging protocols which assess neutrophilic inflammation in the lung.

Aim

To quantify whole lung neutrophil accumulation in (1) healthy volunteers (HV) following inhaled lipopolysaccharide (LPS) or saline and (2) patients with COPD using radiolabelled autologous neutrophils and single-photon emission computed tomography/CT (SPECT/CT).

Methods

20 patients with COPD (Global initiative for chronic obstructive lung disease (GOLD) stages 2-3) and 18 HVs were studied. HVs received inhaled saline (n=6) or LPS (50 µg, n=12) prior to the injection of radiolabelled cells. Neutrophils were isolated using dextran sedimentation and Percoll plasma gradients and labelled with ^99mTechnetium (Tc)-hexamethylpropyleneamine oxime. SPECT was

SUBMITTER: Tregay N 

PROVIDER: S-EPMC6585304 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature