Primer Design for an Accurate View of Picocyanobacterial Community Structure by Using High-Throughput Sequencing.

ABSTRACT: High-throughput sequencing (HTS) of the 16S rRNA gene has been used successfully to describe the structure and dynamics of microbial communities. Picocyanobacteria are important members of bacterioplankton communities, and, so far, they have predominantly been targeted using universal bacterial primers, providing a limited resolution of the picocyanobacterial community structure and dynamics. To increase such resolution, the study of a particular target group is best approached with the use of specific primers. Here, we aimed to design and evaluate specific primers for aquatic picocyanobacterial genera to be used with high-throughput sequencing. Since the various regions of the 16S rRNA gene have different degrees of conservation in different bacterial groups, we therefore first determined

SUBMITTER: Huber P 

PROVIDER: S-EPMC6585506 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature