Biodegradable nanosyringes for intracellular amplification-based dual-diagnosis and gene therapy in single living cells.

ABSTRACT: The efficient delivery of biomolecules into living cells as well as their easy biodegradation have been challenges for the application of intracellular amplification for sensitive multiple-diagnosis and gene therapy for cancer. Herein, new strategies of amplification-based dual-detection of cancer biomarkers (Let-7a miRNA and VEGF) and gene therapy for cancers are put forward. These are achieved through biodegradable nanosyringes (NSs), rigid and sharp in vitro but degradable in vivo, which are applied for the efficient loading, delivery and release of biomolecules (enzymes, nucleic acids, and even silencing RNA) into living cells. After penetrating cell membranes and escaping from endosomes through their rigid and sharp tips, NSs release biomolecules for fast and easy "one-s

SUBMITTER: Shen X 

PROVIDER: S-EPMC6585592 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature