Development of pseudo-linear gradient elution for high-throughput resin selectivity screening in RoboColumn^® Format.

ABSTRACT: Rapid development of chromatographic processes relies on effective high-throughput screening (HTS) methods. This article describes the development of pseudo-linear gradient elution for resin selectivity screening using RoboColumns^® . It gives guidelines for the implementation of this HTS method on a Tecan Freedom EVO^® robotic platform, addressing fundamental aspects of scale down and liquid handling. The creation of a flexible script for buffer preparation and column operation plus efficient data processing provided the basis for this work. Based on the concept of discretization, linear gradient elution was transformed into multistep gradients. The impact of column size, flow rate, multistep gradient design, and fractionation scheme on separation efficiency was system

SUBMITTER: Kiesewetter A 

PROVIDER: S-EPMC6585662 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature