Advanced mare age impairs the ability of in vitro-matured oocytes to correctly align chromosomes on the metaphase plate.

ABSTRACT:

Background

Advanced mare age is associated with declining fertility and an increased risk of early pregnancy loss. Compromised oocyte quality is probably the primary reason for reduced fertility, but the defects predisposing to embryonic death are unknown. In women, advanced age predisposes to chromosome segregation errors during meiosis, which lead to embryonic aneuploidy and a heightened risk of miscarriage.

Objectives

To evaluate the effect of advanced mare age on chromosome alignment and meiotic spindle morphology in in vitro-matured (IVM) oocytes.

Study design

Morphometric and morphological analysis.

Methods

To investigate differences in spindle organisation and chromosome alignment between young and old mares, oocytes collected from slaughtered mares were

SUBMITTER: Rizzo M 

PROVIDER: S-EPMC6585749 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature