A novel enzymatic method for the measurement of lactose in lactose-free products.

ABSTRACT:

Background

In recent years there has been a surge in the number of commercially available lactose-free variants of a wide variety of products. This presents an analytical challenge for the measurement of the residual lactose content in the presence of high levels of mono-, di-, and oligosaccharides.

Results

In the current work, we describe the development of a novel enzymatic low-lactose determination method termed LOLAC (low lactose), which is based on an optimized glucose removal pre-treatment step followed by a sequential enzymatic assay that measures residual glucose and lactose in a single cuvette. Sensitivity was improved over existing enzymatic lactose assays through the extension of the typical glucose detection biochemical pathway to amplify the signal response. Sel

SUBMITTER: Mangan D 

PROVIDER: S-EPMC6585930 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature