Epilepsy-on-a-Chip System for Antiepileptic Drug Discovery.

ABSTRACT:

Objective

Hippocampal slice cultures spontaneously develop chronic epilepsy several days after slicing and are used as an in vitro model of post-traumatic epilepsy. Here, we describe a hybrid microfluidic-microelectrode array (μflow-MEA) technology that incorporates a microfluidic perfusion network and electrodes into a miniaturized device for hippocampal slice culture based antiepileptic drug discovery.

Methods

Field potential simulation was conducted to help optimize the electrode design to detect a seizure-like population activity. Epilepsy-on-a-chip model was validated by chronic electrical recording, neuronal survival quantification, and anticonvulsant test. To demonstrate the application of μflow-MEA in drug discovery, we utilized a two-stage screening platform to iden

SUBMITTER: Liu J 

PROVIDER: S-EPMC6585967 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature