Cannabinoid-1 receptor regulates mitochondrial dynamics and function in renal proximal tubular cells.

ABSTRACT:

Aims

To evaluate the specific role of the endocannabinoid/cannabinoid type-1 (CB₁ R) system in modulating mitochondrial dynamics in the metabolically active renal proximal tubular cells (RPTCs).

Materials and methods

We utilized mitochondrially-targeted GFP in live cells (wild-type and null for the CB₁ R) and electron microscopy in kidney sections of RPTC-CB₁ R^-/- mice and their littermate controls. In both in vitro and in vivo conditions, we assessed the ability of CB₁ R agonism or fatty acid flux to modulate mitochondrial architecture and function.

Results

Direct stimulation of CB₁ R resulted in mitochondrial fragmentation in RPTCs. This process was mediated, at least in part, by modulating the phosph

SUBMITTER: Drori A 

PROVIDER: S-EPMC6586028 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature