Activity of two hyaluronan preparations on primary human oral fibroblasts.

ABSTRACT:

Background and objective

The potential benefit of using hyaluronan (HA) in reconstructive periodontal surgery is still a matter of debate. The aim of the present study was to evaluate the effects of two HA formulations on human oral fibroblasts involved in soft tissue wound healing/regeneration.

Material and methods

Metabolic, proliferative and migratory abilities of primary human palatal and gingival fibroblasts were examined upon HA treatment. To uncover the mechanisms whereby HA influences cellular behavior, wound healing-related gene expression and activation of signaling kinases were analyzed by qRT-PCR and immunoblotting, respectively.

Results

The investigated HA formulations maintained the viability of oral fibroblasts and increased their proliferative and migr

SUBMITTER: Asparuhova MB 

PROVIDER: S-EPMC6586051 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature