High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer.

ABSTRACT: Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses fall below the detection limits of conventional flow cytometers due to the overlap of particle-associated scattered light signals with the detection of background instrument noise from diffusely scattered light. To identify, sort, and study distinct subsets of EVs and other nanoparticles, as individual particles, we developed nanoscale Fluorescence Analysis and Cytometric Sorting (nanoFACS) methods to maximise information and material that can be obtained with high speed, high resolution flow cytometers. This nanoFACS method requires analysis of the instrument background noise (

SUBMITTER: Morales-Kastresana A 

PROVIDER: S-EPMC6586126 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature