Assessment of reproducibility and biological variability of fasting and postprandial plasma metabolite concentrations using 1H NMR spectroscopy.

ABSTRACT:

Introduction

It is crucial to understand the factors that introduce variability before applying metabolomics to clinical and biomarker research.

Objectives

We quantified technical and biological variability of both fasting and postprandial metabolite concentrations measured using 1H NMR spectroscopy in plasma samples.

Methods

In the Netherlands Epidemiology of Obesity study (n = 6,671), 148 metabolite concentrations (101 metabolites belonging to lipoprotein subclasses) were measured under fasting and postprandial states (150 minutes after a mixed liquid meal). Technical variability was evaluated among 265 fasting and 851 postprandial samples, with the identical blood plasma sample being measured twice by the same laboratory protocol. Biological reproducibility was ass

SUBMITTER: Li-Gao R 

PROVIDER: S-EPMC6586348 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature