An integrin α_IIbβ₃ intermediate affinity state mediates biomechanical platelet aggregation.

ABSTRACT: Integrins are membrane receptors that mediate cell adhesion and mechanosensing. The structure-function relationship of integrins remains incompletely understood, despite the extensive studies carried out because of its importance to basic cell biology and translational medicine. Using a fluorescence dual biomembrane force probe, microfluidics and cone-and-plate rheometry, we applied precisely controlled mechanical stimulations to platelets and identified an intermediate state of integrin α_IIbβ₃ that is characterized by an ectodomain conformation, ligand affinity and bond lifetimes that are all intermediate between the well-known inactive and active states. This intermediate state is induced by ligand engagement of glycoprotein (GP) Ibα via a mechanosignalling pathway

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6586518 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature