Isotopic labelling reveals the efficient adaptation of wheat root TCA cycle flux modes to match carbon demand under ammonium nutrition.

ABSTRACT: Proper carbon (C) supply is essential for nitrogen (N) assimilation especially when plants are grown under ammonium (NH₄⁺) nutrition. However, how C and N metabolic fluxes adapt to achieve so remains uncertain. In this work, roots of wheat (Triticum aestivum L.) plants grown under exclusive NH₄⁺ or nitrate (NO₃^-) supply were incubated with isotope-labelled substrates (¹⁵NH₄⁺, ¹⁵NO₃^-, or [¹³C]Pyruvate) to follow the incorporation of ¹⁵N or ¹³C into amino acids and organic acids. Roots of plants adapted to ammonium nutrition presented higher capacity to incorporate both ¹⁵NH₄⁺ and ¹⁵NO<

SUBMITTER: Vega-Mas I 

PROVIDER: S-EPMC6586781 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature