Substrate-induced conformational dynamics of the dopamine transporter.

ABSTRACT: The dopamine transporter is a member of the neurotransmitter:sodium symporters (NSSs), which are responsible for termination of neurotransmission through Na⁺-driven reuptake of neurotransmitter from the extracellular space. Experimental evidence elucidating the coordinated conformational rearrangements related to the transport mechanism has so far been limited. Here we probe the global Na⁺- and dopamine-induced conformational dynamics of the wild-type Drosophila melanogaster dopamine transporter using hydrogen-deuterium exchange mass spectrometry. We identify Na⁺- and dopamine-induced changes in specific regions of the transporter, suggesting their involvement in protein conformational transitions. Furthermore, we detect ligand-dependent slow cooperative fl

SUBMITTER: Nielsen AK 

PROVIDER: S-EPMC6586795 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature