In vitro Cell Migration, Invasion, and Adhesion Assays: From Cell Imaging to Data Analysis.

ABSTRACT: Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation, and cancer progression. How physical, chemical, and molecular aspects can affect cell motility is a challenge to understand migratory cells behavior. In vitro assays are excellent approaches to extrapolate to in vivo situations and study live cells behavior. Here we present four in vitro protocols that describe step-by-step cell migration, invasion and adhesion strategies and their corresponding image data quantification. These current protocols are based on two-dimensional wound healing assays (comparing traditional pipette tip-scratch assay vs. culture insert assay), 2D individual cell-tracking experiments by live cell imaging and

SUBMITTER: Pijuan J 

PROVIDER: S-EPMC6587234 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature