An MLST approach to support tracking of plasmids carrying OXA-48-like carbapenemase.

ABSTRACT:

Objectives

The prevalence of infections caused by OXA-48-like carbapenemase-producing organisms in Ireland has increased dramatically since 2011 and is an urgent public health issue. Genome-based high-resolution genotyping was used to analyse clinical isolates submitted to the Irish Carbapenemase-Producing Enterobacteriaceae Reference Laboratory Service for a 13 month period (2016-17).

Methods

A total of 109 OXA-48-producing non-duplicate clinical isolates from 16 submitting centres were sequenced. Using a gene-by-gene approach, isolate genomes were characterized by MLST and core genome MLST, and the presence of antimicrobial resistance determinants was determined. Reference mapping and a novel plasmid MLST-type approach was applied to determine plasmid background.

Result

SUBMITTER: Brehony C 

PROVIDER: S-EPMC6587408 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature