Ex vivo treatment of prostate tumor tissue recapitulates in vivo therapy response.

ABSTRACT:

Background

In vitro models of prostate cancer (PCa) are not always reliable to evaluate anticancer treatment efficacy. This limitation may be overcome by using viable tumor slice material. Here we report on the establishment of an optimized ex vivo method to culture tissue slices from patient-derived xenografts (PDX) of prostate cancer (PCa), to assess responses to PCa treatments.

Methods

Three PDX models were used that are characterized by different androgen receptor (AR) expression and different homology directed DNA repair capacities, due to a breast cancer associated two (BRCA2) wild-type or mutated status. Tumors were removed from mice, sliced using a vibratome and cultured for a maximum of 6 days. To test the sensitivity to androgen antagonist, tumor slices from the AR

SUBMITTER: Zhang W 

PROVIDER: S-EPMC6587720 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature