Fluorescence lifetime shifts of NAD(P)H during apoptosis measured by time-resolved flow cytometry.

ABSTRACT: Autofluorescence from the intracellular metabolite, NAD(P)H, is a biomarker that is widely used and known to reliably screen and report metabolic activity as well as metabolic fluctuations within cells. As a ubiquitous endogenous fluorophore, NAD(P)H has a unique rate of fluorescence decay that is altered when bound to coenzymes. In this work we measure the shift in the fluorescence decay, or average fluorescence lifetime (1-3 ns), of NAD(P)H and correlate this shift to changes in metabolism that cells undergo during apoptosis. Our measurements are made with a flow cytometer designed specifically for fluorescence lifetime acquisition within the ultraviolet to violet spectrum. Our methods involved culture, treatment, and preparation of cells for cytometry and microscopy measurements. The ev

SUBMITTER: Alturkistany F 

PROVIDER: S-EPMC6587805 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature