Microbiota-Dependent Involvement of Th17 Cells in Murine Models of Inflammatory Arthritis.

ABSTRACT:

Objective

Intestinal microbiota are associated with the development of inflammatory arthritis. The aim of this study was to dissect intestinal mucosal immune responses in the preclinical phase of arthritis and determine whether the presence of Th17 cells, beyond involvement of the cytokine interleukin-17 (IL-17), is required for arthritis development, and whether the involvement of Th17 cells in arthritis depends on the composition of the host microbiota.

Methods

Mucosal T cell production of IL-17, interferon-γ, tumor necrosis factor α (TNFα), IL-22, and granulocyte-macrophage colony-stimulating factor (GM-CSF) was analyzed by flow cytometry and Luminex assay before arthritis onset in mice immunized to develop collagen-induced arthritis (CIA). Pathogenic features of arthriti

SUBMITTER: Evans-Marin H 

PROVIDER: S-EPMC6587816 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature