Tuning of hydrogel stiffness using a two-component peptide system for mammalian cell culture.

ABSTRACT: Self-assembling peptide hydrogels (SAPHs) represent emerging cell cultures systems in several biomedical applications. The advantages of SAPHs are mainly ascribed to the absence of toxic chemical cross-linkers, the presence of ECM-like fibrillar structures and the possibility to produce hydrogels with a large range of different mechanical properties. We will present a two-component peptide system with tuneable mechanical properties, consisting of a small pentapeptide (SFFSF-NH₂ , SA5N) that acts as a gelator and a larger 21-mer peptide (SFFSF-GVPGVGVPGVG-SFFSF, SA21) designed as a physical cross-linker. The hydrogels formed by different mixtures of the two peptides are made up mainly of antiparallel β-sheet nanofibers entangling in an intricate network. The effect of the additio

SUBMITTER: Scelsi A 

PROVIDER: S-EPMC6587839 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature